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State of art and best practices for fatty acid analysis in aquatic sciences
Couturier, L.I.E.; Michel, L.C.; Amaro, T.; Budge, S.M.; da Costa, E.; De Troch, M.; Di Dato, V.; Fink, P.; Giraldo, C.; Le Grand, F.; Loaiza, I.; Mathieu-Resuge, M.; Nichols, P.; Parrish, C.C.; Sardenne, F.; Vagner, M.; Pernet, F.; Soudant, P. (2020). State of art and best practices for fatty acid analysis in aquatic sciences. ICES J. Mar. Sci./J. Cons. int. Explor. Mer 77(7-8): 2375-2395. https://dx.doi.org/10.1093/icesjms/fsaa121
In: ICES Journal of Marine Science. Academic Press: London. ISSN 1054-3139; e-ISSN 1095-9289
Peer reviewed article  
Available in  Authors 
    Vlaams Instituut voor de Zee: Open access 357030 [ download pdf ]
Keywords
    Acids > Organic compounds > Organic acids > Fatty acids
    Biomarkers
    Methods
    Marine/Coastal
Author keywords
    aquatic nutrition, arine and freshwater lipids, recommended practices
Authors  Top 
  • Couturier, L.I.E.
  • Michel, L.C.
  • Amaro, T.
  • Budge, S.M.
  • da Costa, E.
  • De Troch, M.
  • Di Dato, V.
  • Fink, P.
  • Giraldo, C.
  • Le Grand, F.
  • Loaiza, I.
  • Mathieu-Resuge, M.
  • Nichols, P.
  • Parrish, C.C.
  • Sardenne, F.
  • Vagner, M.
  • Pernet, F.
  • Soudant, P.
Abstract
    Determining the lipid content and fatty acid (FA) composition of aquatic organisms has been of major interest in trophic ecology, aquaculture, and nutrition for over half a century. Although protocols for lipid analysis are well-described, their application to aquatic sciences often requires modifications to adapt to field conditions and to sample type. Here, we present the current state of knowledge of methods dedicated to both marine and freshwater lipid analyses, from sampling to data treatment. We review: (i) sample preservation, storage and transport protocols, and their effects on lipids, (ii) lipid extraction, separation of polar and neutral lipids, derivatization, and detection methods, and (iii) available tools for the statistical analysis of FA data. We provide recommendations for best practices in field situations and advocate for protocol standardization and interlaboratory calibration.
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