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Long-term patterns of hydrocarbon biodegradation and bacterial community composition in epipelagic and mesopelagic zones of an Arctic fjord
Kampouris, I.D.; Gründger, G.F.; Christensen, J.H.; Greer, C.W.; Kjeldsen, K.U.; Boone, W.; Meire, L.; Rysgaard, S.; Vergeynst, L. (2023). Long-term patterns of hydrocarbon biodegradation and bacterial community composition in epipelagic and mesopelagic zones of an Arctic fjord. J. Hazard. Mater. 446: 130656. https://dx.doi.org/10.1016/j.jhazmat.2022.130656
In: Journal of hazardous materials. Elsevier: Amsterdam; Lausanne; New York; Oxford; Shannon; Tokyo. ISSN 0304-3894; e-ISSN 1873-3336
Peer reviewed article  
Available in  Authors 
  • Vlaams Instituut voor de Zee: Non-open access 385901 [ request ]
  • Vlaams Instituut voor de Zee: Open Marine Archive 394554 [ download pdf ] Accepted manuscript
  • NIOZ: NIOZ files 387388
Keyword
    Marine/Coastal
Author keywords
    Oil biodegradation; Arctic; Microbiome; Oil-degrading bacteria
Authors  Top 
  • Kampouris, I.D.
  • Gründger, G.F.
  • Christensen, J.H.
  • Greer, C.W.
  • Kjeldsen, K.U.
  • Boone, W.
  • Meire, L.
  • Rysgaard, S.
  • Vergeynst, L.
Abstract
    Oil spill attenuation in Arctic marine environments depends on oil-degrading bacteria. However, the seasonally harsh conditions in the Arctic such as nutrient limitations and sub-zero temperatures limit the activity even for bacteria capable of hydrocarbon metabolism at low temperatures. Here, we investigated whether the variance between epipelagic (seasonal temperature and inorganic nutrient variations) and mesopelagic zone (stable environmental conditions) could limit the growth of oil-degrading bacteria and lead to lower oil biodegradation rates in the epipelagic than in the mesopelagic zone. Therefore, we deployed absorbents coated with three oil types in a SW-Greenland fjord system at 10–20 m (epipelagic) and 615–650 m (mesopelagic) water depth for one year. During this period we monitored the development and succession of the bacterial biofilms colonizing the oil films by 16S rRNA gene amplicon quantification and sequencing, and the progression of oil biodegradation by gas chromatography – mass spectrometry oil fingerprinting analysis. The removal of hydrocarbons was significantly different, with several polycyclic aromatic hydrocarbons showing longer half-life times in the epipelagic than in the mesopelagic zone. Bacterial community composition and density (16S rRNA genes/ cm2) significantly differed between the two zones, with total bacteria reaching to log-fold higher densities (16S rRNA genes/cm2) in the mesopelagic than epipelagic oil-coated absorbents. Consequently, the environmental conditions in the epipelagic zone limited oil biodegradation performance by limiting bacterial growth.
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